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Selected Publication:

Type of publication: Journal Article
Type of document: Full Paper

Year: 2016

Authors: Mann, E; Wetzels, SU; Pinior, B; Metzler-Zebeli, BU; Wagner, M; Schmitz-Esser, S

Title: Psychrophile spoilers dominate the bacterial microbiome in musculature samples of slaughter pigs.

Source: Meat Sci. 2016; 117:36-40

Authors Vetmeduni Vienna:

Conrady Beate
Metzler-Zebeli Barbara
Schmitz-Esser Stephan
Selberherr Evelyne
Wagner Martin
Wetzels Stefanie

Vetmed Research Units
Institute of Animal Nutrition and Functional Plant Compounds
Institute of Food Safety, Food Technology and Veterinary Public Health, Unit of Food Microbiology
Institute of Food Safety, Food Technology and Veterinary Public Health, Unit of Veterinary Public Health and Epidemiology
University Clinic for Swine

Project(s): d-i.INFLACOW: Characterization and prevention of diet-induced inflammation and related immune and metabolic disorders in dairy cows

Genetic and cytogenetic bases of Cardinium-caused cytoplasmic incompatibility

Characterization of the gut microbiota in cattle in terms of food quality and animal health

The aim of this study was to disentangle the microbial diversity on porcine musculature. The hypervariable V1-V2 region of the 16S rRNA gene was amplified from DNA samples of clinically healthy slaughter pigs (n=8). Pyrosequencing yielded 37,000 quality-controlled reads and a diverse microbiome with 54-159 OTUs per sample was detected. Interestingly, 6 out of 8 samples were strongly dominated by 1-2 highly abundant OTUs (best hits of highly abundant OTUs: Serratia proteamaculans, Pseudomonas syringae, Aeromonas allosaccharophila, Brochothrix thermosphacta, Acidiphilium cryptum and Escherichia coli). In 1g musculature scraping, 3.20E+06 16S rRNA gene copies and 4.45E+01 Enterobacteriaceae rRNA gene copies were detected with qPCR. We conclude that i.) next-generation sequencing technologies help encompass the full content of complex, bacterial contamination, ii.) psychrophile spoilers dominated the microbiota and iii.) E. coli is an effective marker species for pork contamination, as it was one of very few abundant species being present in all samples. Copyright © 2016 Elsevier Ltd. All rights reserved.

Keywords Pubmed: Abattoirs
Bacteriaclassificationgeneticsisolation & purification
Muscle, Skeletalmicrobiology
RNA, Bacterialgenetics
RNA, Ribosomal, 16Sgenetics

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