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Type of publication: Journal Article
Type of document: Full Paper

Year: 2018

Authors: Herrmann, I; Gotovina, J; Fazekas-Singer, J; Fischer, MB; Hufnagl, K; Bianchini, R; Jensen-Jarolim, E

Title: Canine macrophages can like human macrophages be in vitro activated toward the M2a subtype relevant in allergy.

Source: Dev Comp Immunol. 2018; 82:118-127

Authors Vetmeduni Vienna:

Bianchini Rodolfo
Gotovina Jelena
Herrmann Ina
Hufnagl Karin
Jensen-Jarolim Erika
Singer Judit

Vetmed Research Units
Messerli Research Institute, Comparative Medicine

Project(s): Cell communication in health and disease

Development of mimotope vaccines for preclinical and comparative medicine studies

The M2a subtype of macrophages plays an important role in human immunoglobulin E (IgE-mediated allergies) and other Th2 type immune reactions. In contrast, very little is known about these cells in the dog. Here we describe an in vitro method to activate canine histiocytic DH82 cells and primary canine monocyte-derived macrophages (MDMs) toward the M2a macrophages using human cytokines. For a side-by-side comparison, we compared the canine cells to human MDMs, and the human monocytic cell line U937 activated towards M1 and M2a cells on the cellular and molecular level. In analogy to activated human M2a cells, canine M2a, differentiated from both DH82 and MDMs, showed an increase in CD206 surface receptor expression compared to M1. Interestingly, canine M2a, but not M1 derived from MDM, upregulated the high-affinity IgE receptor (FcεRI). Transcription levels of M2a-associated genes (IL10, CCL22, TGFβ, CD163) showed a diverse pattern between the human and dog species, whereas M1 genes (IDO1, CXCL11, IL6, TNF-α) were similarly upregulated in canine and human M1 cells (cell lines and MDMs). We suggest that our novel in vitro method will be suitable in comparative allergology studies focussing on macrophages.Copyright © 2018 The Authors. Published by Elsevier Ltd.. All rights reserved.

Keywords Pubmed: Animals
Cell Differentiationgenetics
Lectins, C-Typemetabolism
Macrophage Activation
Mannose-Binding Lectinsmetabolism
Receptors, Cell Surfacemetabolism
Receptors, IgEmetabolism
Species Specificity
Th2 Cellsimmunology
U937 Cells

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