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Type of publication: Journal Article
Type of document: Full Paper

Year: 2018

Authors: Kumar, G; Hummel, K; Noebauer, K; Welch, TJ; Razzazi-Fazeli, E; El-Matbouli, M

Title: Proteome analysis reveals a role of rainbow trout lymphoid organs during Yersinia ruckeri infection process.

Source: Sci Rep. 2018; 8(1):13998


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VetCore (Proteomics);

Authors Vetmeduni Vienna:

El-Matbouli Mansour
Hummel Karin
Kumar Gokhlesh
Nöbauer Katharina
Razzazi-Fazeli Ebrahim

Vetmed Research Units
University Clinic for Poultry and Fish Medicine, Clinical Unit of Fish Medicine
VetCore


Project(s): Proteomics of rainbow trout in Response to Yersinia ruckeri


Abstract:
Yersinia ruckeri is the causative agent of enteric redmouth disease in salmonids. Head kidney and spleen are major lymphoid organs of the teleost fish where antigen presentation and immune defense against microbes take place. We investigated proteome alteration in head kidney and spleen of the rainbow trout following Y. ruckeri strains infection. Organs were analyzed after 3, 9 and 28 days post exposure with a shotgun proteomic approach. GO annotation and protein-protein interaction were predicted using bioinformatic tools. Thirty four proteins from head kidney and 85 proteins from spleen were found to be differentially expressed in rainbow trout during the Y. ruckeri infection process. These included lysosomal, antioxidant, metalloproteinase, cytoskeleton, tetraspanin, cathepsin B and c-type lectin receptor proteins. The findings of this study regarding the immune response at the protein level offer new insight into the systemic response to Y. ruckeri infection in rainbow trout. This proteomic data facilitate a better understanding of host-pathogen interactions and response of fish against Y. ruckeri biotype 1 and 2 strains. Protein-protein interaction analysis predicts carbon metabolism, ribosome and phagosome pathways in spleen of infected fish, which might be useful in understanding biological processes and further studies in the direction of pathways.

Keywords Pubmed: Animals
Fish Diseasesmetabolismmicrobiology
Head Kidneymetabolism
Oncorhynchus mykissgrowth & developmentmetabolismmicrobiology
Proteomeanalysis
Spleenmetabolism
Yersinia Infectionsmetabolismmicrobiologyveterinary
Yersinia ruckeriphysiology

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