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Selected Publication:

Type of publication: Journal Article
Type of document: Full Paper

Year: 2020

Authors: Wenning, M; Breitenwieser, F; Huptas, C; Doll, E; Bächler, B; Schulz, A; Dunkel, A; Hofmann, T; von Neubeck, M; Busse, HJ; Scherer, S

Title: Brevilactibacter flavus gen. nov., sp. nov., a novel bacterium of the family Propionibacteriaceae isolated from raw milk and dairy products and reclassification of Propioniciclava sinopodophylli as Brevilactibacter sinopodophylli comb. nov.

Source: Int J Syst Evol Microbiol. 2020; 70(4):2186-2193



Authors Vetmeduni Vienna:

Busse Hans-Jürgen

Vetmed Research Units
Institute of Microbiology


Abstract:
Eight facultatively anaerobic rod-shaped bacteria were isolated from raw milk and two other dairy products. Results of phylogenetic analyses based on 16S rRNA gene sequences showed that the isolates are placed in a distinct lineage within the family Propionibacteriaceae with Propioniciclava sinopodophylli and Propioniciclava tarda as the closest relatives (94.6 and 93.5 % similarity, respectively). The cell-wall peptidoglycan contained meso-diaminopimelic acid, alanine and glutamic acid and was of the A1γ type (meso-DAP-direct). The major cellular fatty acid was anteiso-C15 : 0 and the major polar lipids were diphosphatidylglycerol, phosphatidyglycerol and three unidentified glycolipids. The quinone system contained predominantly menaquinone MK-9(H4). The G+C content of the genomic DNA of strain VG341T was 67.7 mol%. The whole-cell sugar pattern contained ribose, rhamnose, arabinose and galactose. On the basis of phenotypic and genetic data, eight strains (VG341T, WS4684, WS4769, WS 4882, WS4883, WS4901, WS4902 and WS4904) are proposed to be classified as members of a novel species in a new genus of the family Propionibacteriaceae, for which the name Brevilactibacter flavus gen. nov., sp. nov. is proposed. The type strain is VG341T (=WS4900T=DSM 100885T=LMG 29089T) and seven additional strains are WS4684, WS4769, WS4882, WS4883, WS4901, WS4902 and WS4904. Furthermore, we propose the reclassification of P. sinopodophylli as Brevilactibacter sinopodophylli comb. nov.

Keywords Pubmed: Animals
Bacterial Typing Techniques
Base Composition
Cell Wallchemistry
DNA, Bacterialgenetics
Dairy Productsmicrobiology
Diaminopimelic Acidchemistry
Fatty Acidschemistry
Food Microbiology
Germany
Glycolipidschemistry
Milkmicrobiology
Peptidoglycanchemistry
Phospholipidschemistry
Phylogeny
Propionibacteriaceaeclassificationisolation & purification
RNA, Ribosomal, 16Sgenetics
Sequence Analysis, DNA
Vitamin K 2analogs & derivativeschemistry

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