University of Veterinary Medicine Vienna - Research portal

Selected Publication:

Open Access Logo

Publication type: Baccalaureate Thesis

Year: 2012

Author(s): Chen, Quan

Title: Gytotoxic/static properties of Cordyceps sinensis extracts and Cordycepin in neuronal tumor cell lines.

Other title: Zytotoxische/Statistische Eigenschaften von Cordyceps Sinensis Extrakten und Cordycepin in neuronalen Tumor Zelllinien

Source: Bakkalaureatsarbeit, Vet. Med. Univ. Wien, pp. 43.


Advisor(s):

Rausch Wolf-Dieter

Reviewer(s):
Möstl Erich

Vetmed Research Units:
Institute for Medical Biochemistry


Graduation date: 08.10.2012


Abstract:
In the recent studies, it was believed that extracts of Cordyceps sinensis and Cordycepin (3'-deoxyadenosine) have anticancer activity, which was based on their antiproliferative and cytotoxic effect. Extracts of Cordyceps sinensis and Cordycepin have already been tested on many different cancer cell lines (mouse melanoma cell line, mouse lung carcinoma cell line and human oral squamous cancer cell line); it showed that extracts of Cordyceps sinensis and Cordycepin can induce programmed cell death (Apoptosis). Therefore it is hypothesized that such antitumor effects do exist also to neural tumors. To prove this hypothesis these compounds were be added to well characterize NB cell line (N18TG2) and their cytostatic effects were be tested dose/time dependently. In this study, an attempt was made to elucidate a water extract of Cordyceps sinensis mycelium, on the growth curves of N18TG2 cells (with and without treatment with water extract of Cordyceps sinensis mycelium). MTT-assay and PI staining were used to investigate the effect of Cordyceps sinensis water extract and Cordycepin on cell viability in N18TG2 cells. Data was presented as concentration and time dependent inhibition curves with statistic calculations. By the cell culture, we have seen the morphological changes of cells like rounded-up phenomenon, floated cells, membrane blebbing and cell death with the treatment of Cordycepin at 1 mM but we did not see these morphological changes by the treatment with Cordyceps sinensis mycelium water extract. MTT assay was used to investigate the effect of Cordyceps sinensis water extract and Cordycepin on cell viability in N18TG2 cells. Results showed that treatment with Cordyceps sinensis mycelium water extract at the final concentration of 2.3 mg/ml and 0.23 mg/ml had significantly decrease in cell number of 38% and 16%. Treatment with Cordycepin at the final concentration of (1 mM, 0.1mM and 0.01 mM) had significantly negative effect on cell viability in N18TG2 cells, significantly decrease in cell number of 85%, 72% and 56% (Fig.22 and Fig.23, * p<0.05). PI staining showed also that Cordycepin at the final concentration of (0.01mM, 0.1mM and 1mM) had significantly cytotoxic effect on neuronal tumor cells. So it can be concluded that Cordycepin pure has cytotoxic effect at 1 mM, 0.1 mM and 0.01 mM but Cordyceps sinensis water extract have only inhibition effect on the N18TG2 cell proliferation (cytostatic effect).


© University of Veterinary Medicine ViennaHelp and Downloads