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Publication type: Journal Article
Document type: Full Paper

Year: 2010

Author(s): Yunus, AW; Awad, WA; Kröger, S; Zentek, J; Böhm, J

Title: In vitro aflatoxin B(1) exposure decreases response to carbamylcholine in the jejunal epithelium of broilers.

Source: Poult Sci. 2010; 89(7):1372-1378



Authors Vetmeduni Vienna:

Awad Wageha
Böhm Josef

Vetmed Research Units
Institute of Animal Nutrition and Functional Plant Compounds


Abstract:
The study was conducted to evaluate if aflatoxin B(1) (AFB(1)) has the capacity to affect the electrophysiological variables and active glucose uptake in jejunal epithelium of chicken. For this purpose, intestinal segments from the middle jejunum of broilers (35 to 39 d old) were incubated in Ussing chambers in the presence of 0 (vehicle control), 1.25, 2.50, and 3.75 microg of AFB(1)/mL of buffer. After 40 and 60 min of incubation with AFB(1), d-glucose (20 mmol/L) and carbamylcholine (200 micromol/L; an analog of acetylcholine and inducer of apical Cl(-) secretion) were respectively added to the incubation medium. Addition of 3.75 microg of AFB(1) caused an increase (P < 0.04) in short-circuit current (I(sc)) and transmural potential difference (V(t)) between 12 to 27 min postexposure as compared with the control. Glucose-induced DeltaI(sc) and percentage of DeltaV(t) were reduced (P < 0.04) at 2.5 and 3.75 microg of AFB(1)/mL, respectively, as compared with the control. The carbamylcholine-induced DeltaI(sc) and DeltaV(t) were both lower (P < 0.05) at 3.75 microg of AFB(1)/mL as compared with the control (-0.05 microA/cm(2), 0.1 mV vs. 1.1 microA/cm(2), and 0.6 mV, respectively). These observations indicate that acute exposure to AFB(1) may increase apical anion secretion in the jejunal epithelium of chicken. The negative effect of this increased anion secretion on active glucose uptake was, however, not prominent and may be considered as moderate or progressive in nature.

Keywords Pubmed: Aflatoxin B1/pharmacology*
Animals
Carbachol/pharmacology*
Chickens/metabolism*
Drug Interactions
Electrophysiology/methods
Glucose/pharmacokinetics
Intestinal Mucosa/drug effects
Intestinal Mucosa/metabolism
Jejunum/drug effects*
Jejunum/metabolism
Patch-Clamp Techniques/veterinary


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