The following pilot study is a close analysis of sweat, which has been extracted from horses
fed with a dietary supplement containing 25 % of garlic powder. The sweat samples
examined in this study have been obtained from 10 Quarter Horses that were chosen as
suitable for the study after fulfilling a number of criteria, such as, for example the common
similarity in sweat secretion.
The objective of this study is
the confirmation of the metabolites of allicin after feeding the
horses with a supplement “Garlic Special“ produced by the company MIKROP
The first samples were taken before the launch of the project. The following samples were
extracted after a three-week feeding process. The dosage of the used supplement was
indicated on the product. The accurate feeding time had to be inquired personally at the
producer of the garlic powder containing feed.
After extraction, the samples were frozen. In
order to proceed with
the analysis procedure,
the samples have been subsequently thawed, mixed with a solvent (Dichlormethan), cleaned
and finally analyzed through GC-MS.
There were no allicinmetabolites to see in any
of the samples after evaluation of the gas
chromatograms. In order to improve the quantitative analyzability, a pure garlic sample has
been prepared. This sample was used as a positive control of the applied method. After the
analysis of this sample, clear peaks of dithiins could be observed on the chromatogram.
These sulfur compounds are one of the odor materials of garlic. The negative result of sweat
samples resulted into an additional analysis of the feed supplement itself. This step has not
been taken earlier, because the premise of the
study was based primarily on the statement
of the producer. No odor materials or other sulfur compounds were found in the pure sample
of the supplement.
The positive outcome of the pure garlic sample,
where the odor materials were found, leads
to a conclusion that the applied method is suitable for this kind of analysis. Consequently, the
negative outcomes of the sweat samples must be depended on other aspects.
To verify the outcomes of this study, it is necessary to carry out an additional trial. This
experiment would require that the horses have to
be fed with a fudder or dietary supplement
in which the allicinmetabolites can be analyzed using the selected an
However, it is important to pay attention
to the toxic potential of garlic for horses.