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Publication type: Diploma Thesis

Year: 2013

Author(s): Höcher, Martin

Title: Herstellung und Einsatz einer stallspezifischen Streptokokkus suis-Vakzine in einem Zuchtsauenbetrieb mit Streptokokkenproblemen in der Aufzucht.

Other title: Production and usage of a stable specific Streptococcus suis vaccine in a breeding sow stable with streptococcus suis problems during the nursery

Source: Diplomarbeit, Vet. Med. Univ. Wien, pp. 45.


Advisor(s):

Hennig-Pauka Isabel

Reviewer(s):
Saalmüller Armin

Vetmed Research Units:
University Clinic for Swine


Graduation date: 26.06.2013


Abstract:
A Streptococcus suis (S.suis) serotype 7 infection in an Austrian piglet producing farm with 1500 sows is described. Cleaning and disinfection protocols were at high standard and a clear separation between all age groups was guaranteed Newborn piglets were treated parenterally with ceftiofuro n the 1" day of live and witha n1oxicilline for ten days in medicated food after weaning, routinely. \Vhen symptoms reoccured, treatruent with amoxicillinew as continued. In a first step the actual situation was described. Piglets of the control group (Group A, n=979) were not treated with antibiotics while piglets of the treated group received amoxicillin (Group B, n=l012). In the control group a higher number of pigs developed meningitis (1.8%) and arthritis (3.3%) at five to eight weeks of age compared to the treated group (meningitis 0.8%, arthritis1 .1%). Mortality rate was significantly higher in the control group (1.8%) than in the treated group (0.8%). Additional symptoms typical for S.suis infection as endocarditis, wasting, pneumonia or polyserositis were not seen on the farm. Brain and joint samples of untreated, euthanized piglets out of the control group (n= 17) with symptoms of meningitis and/or arthritisw ere examined bacteriologically. Twenty-five S.suis isolates out of 18 piglets (medium to high-grade bacterial growth) were recovered, 17 of them were serotyped by coagglutination technique resulting in serotype 7 in all samples. All isolates were tested by multiplex-PCR using primer pairs specific for virulence-associated genes mrp (muraminidase- released protein), ef (extracellular factor), ofs (serum opacity factor) and sly (suilysin). All isolates were positive for mrp, but negative for epf, ofs and sly. No other pathogens typical for causing meningitis or arthritis could be isolated from clinical samples. Five serotype 7 isolates were chosen for farm-specific vaccine production, which was applicated to suckling piglets in the first and third week of life, but without a protective effect. This case showed, that not only Ssuis serotype 2 (most common serotype in Austria), but also serotype 7 can cause clinical disease in Austrian pig farms. A vaccination program must be adjusted to specific farm conditions taking a potential interference with maternal antibodies and the time-point of outbreak of clinical disease into account.


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