Embryo transfer in mice is a crucial technique for several applications such as
generation of genetically modified rodents, revitalisation of cryopreserved strains and
rederivation of contaminated strains. Recently, a new method for non-surgical
embryo transfer (NSET) in mice was introduced. It eliminates the impact of
anaesthesia and surgery on the embryo recipient potentially without reducing the
success rate. We tested the practicality of this method as determined by pregnancy
rate, number of pups born, and number of implantation sites in laboratory mice.