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Selected Publication:

Type of publication: Journal Article
Type of document: Full Paper

Year: 2000

Authors: Reichart, U; Kappler, R; Scherthan, H; Wolf, E; Müller, M; Brem, G; Aigner, B

Title: Partial leptin receptor gene deletion in transgenic mice prevents expression of the membrane-bound isoforms except for Ob-Rc.

Source: Biochem Biophys Res Commun. 2000; 269(2):496-501

Authors Vetmeduni Vienna:

Aigner Bernhard
Brem Gottfried
Müller Mathias
Reichart Ursula

Vetmed Research Units
Institute of Animal Breeding and Genetics

Examination of random insertional mutations in transgenic animals harbouring an abnormal phenotype contributes to the discovery of new genes and/or the understanding of already known genes. Here we describe a transgenic mouse line showing early-onset obesity as consequence of the transgene insertion. Molecular genetic analysis revealed a partial deletion of the leptin receptor (Lepr, Ob-R) gene including the coding sequences downstream of exon 17". This defect prevents the expression of all described membrane-bound isoforms of Ob-R except for isoform Ob-Rc in the homozygous transgenic animals. Thus, this mouse model might be useful for the investigation of the function of the short Ob-R isoforms.

Keywords Pubmed: Animals
Carrier Proteins/genetics*
Gene Deletion*
Mice, Transgenic
Mutagenesis, Insertional
Protein Isoforms/genetics*
RNA, Messenger/genetics
Receptors, Cell Surface*
Receptors, Leptin

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