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Selected Publication:

Type of publication: Journal Article
Type of document: Full Paper

Year: 1998

Authors: Steinborn, R; Zakhartchenko, V; Jelyazkov, J; Klein, D; Wolf, E; Müller, M; Brem, G

Title: Composition of parental mitochondrial DNA in cloned bovine embryos.

Source: FEBS Lett. 1998; 426(3):352-356

Authors Vetmeduni Vienna:

Brem Gottfried
Klein Dieter
Müller Mathias
Steinborn Ralf

Vetmed Research Units
Department for Agrobiotechnology (IFA Tulln)
Institute of Animal Breeding and Genetics
Institute of Virology

Project(s): Genome Regulation and Gene Therapy in Human and Veterinary Medicine

We have investigated parental mitochondrial DNA (mtDNA) in cloned bovine embryos obtained by intraspecific cytoplast-blastomere fusion. Analysis of two-cell to blastocyst stage embryos revealed that in contrast to the exclusion of paternal (sperm) mtDNA during sexual inheritance in the cytoplast-blastomere fusion complexes, there was mixing and co-existence of parental mtDNA. The mixing of mtDNA was non-balanced with the minority deriving from the blastomere. The constant content of mtDNA during embryogenesis until the blastocyst stage suggesting an absence of mtDNA replication was shown for conventional "in vitro fertilised" (IVF) embryos and for cloned embryos. The ratio of parental mtDNA was in accordance with the estimated quantitative participation of mtDNA from the fusion partners.

Keywords Pubmed: Animals
Cloning, Organism/veterinary*
DNA, Mitochondrial/genetics*
DNA, Mitochondrial/isolation & purification*
Embryo Transfer/veterinary
Extrachromosomal Inheritance/genetics
Fertilization in Vitro/veterinary
Molecular Sequence Data
Polymerase Chain Reaction/veterinary

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