University of Veterinary Medicine Vienna - Research portal

Selected Publication:

Publication type: Journal Article
Document type: Full Paper

Year: 1998

Author(s): Besenfelder, U; Brem, G

Title: Tubal transfer of bovine embryos: a simple endoscopic method reducing long-term exposure of in vitro produced embryos.

Source: Theriogenology (50), 5 739-745.



Authors Vetmeduni Vienna:

Besenfelder Urban
Brem Gottfried

Vetmed Research Units
Institute of Animal Breeding and Genetics


Abstract:
Although numerous trials had shown the need to define a procedure to get free access to the bovine oviduct, there was no adequate report of a technique which was accepted for the routine transfer of early tubal-stage embryos. We have now report an endoscopically mediated transvaginal method for transferring embryos into the oviduct. The in vitro produced embryos were loaded into a curved glass capillary tube which was connected to a perfusor tube plus 1-mL syringe. The capillary tube was directly inserted via the infundibulum into the ampulla. After first having checked the ovaries for the presence of a corpus luteum the embryos were deposited under visual guidance in about 20 to 50 mu L medium. Twenty-four Simmental and Brown Swiss heifers received 26 embryos and 9 animals became pregnant, of which 7 recipients delivered 8 live calves. With practice, the time used for endoscopic transfer was reduced to less than 10 min. The results demonstrate that the described technique is suitable fbr practical application. Especially for the early transfer of IVP-derived embryos this technique might be advantageous. In conclusion, this method is also of great potential interest for the recovery of tubal-stage embryos and for the in vivo culture of embryos followed by conventional flushing at Day 7. (C) 1998 by Elsevier Science Inc.

Keywords Pubmed: Animals - : Cattle - : Embryo Transfer - methods : Endoscopy - veterinary : Fallopian Tubes - veterinary : Female - veterinary : Fertilization in Vitro - veterinary : Male - veterinary : Pregnancy - veterinary : Pregnancy Outcome - veterinary

© University of Veterinary Medicine ViennaHelp and Downloads