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Selected Publication:

Publication type: Journal Article
Document type: Full Paper

Year: 1998

Author(s): Kolb, AF; Günzburg, WH; Brem, G; Erfle, V; Salmons, B

Title: A functional eukaryotic promoter is contained within the first intron of the hGH-N coding region.

Source: Biochem Biophys Res Commun (247), 2 332-337.

Authors Vetmeduni Vienna:

Brem Gottfried
Günzburg Walter

Vetmed Research Units
Institute of Animal Breeding and Genetics
Institute of Virology

Human growth hormone is frequently used as a reporter gene in studies addressing the regulation of eukaryotic promoters. Here we present evidence that the first intron of the hGH-N coding region contains a novel eukaryotic promoter which is able to direct the expression of hGH-N and luciferase reporter genes. The corresponding transcriptional initiation site is located downstream of the splice acceptor site of exon 2. This internal hGH-N promoter can interfere with the transcriptional control elements of a promoter linked to the hGH-N coding region and thereby complicate the interpretation of data obtained with hGH-N as a reporter gene. (C) 1998 Academic Press.

Keywords Pubmed: Animals - : Base Sequence - : Cats - : Cell Line - : DNA Primers - genetics : Gene Expression - genetics : Genes, Reporter - genetics : Human Growth Hormone - genetics : Humans - genetics : Introns - genetics : Luciferases - genetics : Promoter Regions, Genetic - genetics : Transfection - genetics

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