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Selected Publication:

Publication type: Journal Article
Document type: Full Paper

Year: 1992

Author(s): Brenig, B; Brem, G

Title: Genomic organization and analysis of the 5' end of the porcine ryanodine receptor gene (ryr1).

Source: FEBS Lett (298), 2-3 277-279.

Authors Vetmeduni Vienna:

Brem Gottfried

In this study we describe the isolation of genomic clones of the 5' region of the porcine ryanodine receptor gene, a candidate for malignant hyperthermia in pigs and humans. The recombinants were isolated from a porcine liver, genomic DNA library in phage EMBL3A after screening with PCR amplified DNA fragments. The exon/intron structure of the ryanodine receptor gene was determined by DNA sequencing. Based on the sequence data it was possible to develop a simple test for the detection of malignant hyperthermia susceptible and normal pigs.

Keywords Pubmed: Animals - : Base Sequence - : Calcium Channels - genetics : DNA - genetics : Humans - genetics : Malignant Hyperthermia - genetics : Molecular Sequence Data - genetics : Polymerase Chain Reaction - veterinary : Receptors, Cholinergic - genetics : Restriction Mapping - genetics : Ryanodine Receptor Calcium Release Channel - genetics : Sequence Homology, Nucleic Acid - genetics : Swine - genetics : Swine Diseases - genetics

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