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Type of publication: Journal Article
Type of document: Full Paper

Year: 2011

Authors: Webel, R; Milbradt, J; Auerochs, S; Schregel, V; Held, C; Nöbauer, K; Razzazi-Fazeli, E; Jardin, C; Wittenberg, T; Sticht, H; Marschall, M

Title: Two isoforms of the protein kinase pUL97 of human cytomegalovirus are differentially regulated in their nuclear translocation.

Source: J Gen Virol. 2011; 92(Pt 3):638-649

Authors Vetmeduni Vienna:

Nöbauer Katharina
Razzazi-Fazeli Ebrahim

Vetmed Research Units

The pUL97 protein kinase encoded by human cytomegalovirus is a multifunctional determinant of the efficiency of viral replication and phosphorylates viral as well as cellular substrate proteins. Here, we report that pUL97 is expressed in two isoforms with molecular masses of approximately 90 and 100 kDa. ORF UL97 comprises an unusual coding strategy in that five in-frame ATG start codons are contained within the N-terminal 157 aa. Site-directed mutagenesis, transient expression of point and deletion mutants and proteomic analyses accumulated evidence that the formation of the large and small isoforms result from alternative initiation of translation, with the start points being at amino acids 1 and 74, respectively. In vitro kinase assays demonstrated that catalytic activity, in terms of autophosphorylation and histone substrate phosphorylation, was indistinguishable for the two isoforms. An analysis of the intracellular distribution of pUL97 by confocal laser-scanning microscopy demonstrated that both isoforms have a pronounced nuclear localization. Surprisingly, mapping experiments performed to identify the nuclear localization signal (NLS) of pUL97 strongly suggest that the mechanism of nuclear transport is distinct for the two isoforms. While the extreme N terminus (large isoform) comprises a highly efficient, bipartite NLS (amino acids 6-35), a second sequence apparently conferring a less efficient mode of nuclear translocation was identified downstream of amino acid 74 (small and large isoforms). Taken together, the findings argue for a complex mechanism of nuclear translocation for pUL97 which might be linked with fine-regulatory differences between the two isoforms.

Keywords Pubmed: Active Transport, Cell Nucleus
Amino Acid Sequence
Amino Acid Substitution/genetics
Cell Nucleus/chemistry
Cell Nucleus/metabolism*
Cells, Cultured
Codon, Initiator
Microscopy, Confocal
Molecular Sequence Data
Molecular Weight
Mutagenesis, Site-Directed
Nuclear Localization Signals
Phosphotransferases (Alcohol Group Acceptor)/chemistry
Phosphotransferases (Alcohol Group Acceptor)/metabolism*
Protein Isoforms/chemistry
Protein Isoforms/metabolism
Protein Kinases/chemistry
Protein Kinases/metabolism
Protein Transport
Sequence Deletion

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