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Selected Publication:

Publication type: Journal Article
Document type: Full Paper

Year: 2011

Author(s): Kopka, J; Leder, M; Jaureguiberry, SM; Brem, G; Boselli, GO

Title: New optimized DNA extraction protocol for fingerprints deposited on a special self-adhesive security seal and other latent samples used for human identification.

Source: J Forensic Sci. 2011; 56(5):1235-1240

Authors Vetmeduni Vienna:

Brem Gottfried

Vetmed Research Units
Institute of Animal Breeding and Genetics

Obtaining complete short tandem repeat (STR) profiles from fingerprints containing minimal amounts of DNA, using standard extraction techniques, can be difficult. The aim of this study was to evaluate a new kit, Fingerprint DNA Finder (FDF Kit), recently launched for the extraction of DNA and STR profiling from fingerprints placed on a special device known as Self-Adhesive Security Seal Sticker(®) and other latent fingerprints on forensic evidentiary material like metallic guns. The DNA extraction system is based on a reversal of the silica principle, and all the potential inhibiting substances are retained on the surface of a special adsorbent, while nucleic acids are not bound and remain in solution dramatically improving DNA recovery. DNA yield was quite variable among the samples tested, rendering in most of the cases (>90%) complete STR profiles, free of PCR inhibitors, and devoid of artifacts. Even samples with DNA amount below 100 pg could be successfully analyzed.

Keywords Pubmed: Adhesives*
DNA/isolation & purification*
DNA Fingerprinting/instrumentation*
DNA Fingerprinting/methods
Microsatellite Repeats
Polymerase Chain Reaction

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