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Selected Publication:

Publication type: Journal Article
Document type: Full Paper

Year: 2014

Author(s): Sedlak, C; Patzl, M; Saalmüller, A; Gerner, W

Title: IL-12 and IL-18 induce interferon-γ production and de novo CD2 expression in porcine γδ T cells.

Source: Dev Comp Immunol. 2014; 47(1):115-122



Authors Vetmeduni Vienna:

Gerner Wilhelm
Patzl Martina
Saalmüller Armin
Sedlak Corinna

Vetmed Research Units
Institute of Immunology


Project(s): Preventive Veterinary Medicine


Abstract:
γδ T cells are highly abundant in the blood and spleen of pigs but little is known about their functional differentiation. In this study the potential of the type-1 polarizing cytokines IL-12 and IL-18 in combination with IL-2 and Concanavalin A (ConA) to stimulate porcine γδ T cells was investigated. Stimulation of purified γδ T cells with ConA and IL-2 induced a strong proliferation of CD2(-) γδ T cells, whereas additional stimulation with IL-12 and IL-18 caused a stronger proliferation of CD2(+) γδ T cells. IFN-γ could only be detected in supernatants of γδ T-cell cultures supplemented with IL-12 and IL-18. Experiments with sorted CD2/SWC5-defined γδ T-cell subsets revealed that CD2(+)SWC5(-) γδ T cells are the main producers of IFN-γ following stimulation with IL-2/IL-12/IL-18. Additional stimulation with ConA led to an upregulation of CD2 within the CD2(-) γδ T cell subsets, indicating a previously unnoticed plasticity of CD2-defined γδ T cell subsets.

Keywords Pubmed: Animals
Antigens, CD2/genetics*
Cell Proliferation*
Interferon-gamma/genetics*
Interleukin-12/metabolism*
Interleukin-18/metabolism*
Sus scrofa/immunology*
T-Lymphocyte Subsets/cytology*
T-Lymphocyte Subsets/immunology
T-Lymphocyte Subsets/metabolism*


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