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Type of publication: Journal Article
Type of document: Full Paper

Year: 2016

Authors: Hilse, KE; Kalinovich, AV; Rupprecht, A; Smorodchenko, A; Zeitz, U; Staniek, K; Erben, RG; Pohl, EE

Title: The expression of UCP3 directly correlates to UCP1 abundance in brown adipose tissue.

Source: Biochim Biophys Acta. 2016; 1857(1):72-78

Authors Vetmeduni Vienna:

Erben Reinhold
Hilse-Koller Karolina
Pohl Elena
Rupprecht Anne
Smorodchenko Alina
Staniek Katrin
Zeitz Ute

Vetmed Research Units
Institute of Pharmacology and Toxicology
Institute of Physiology, Pathohysiology and Biophysics, Unit of Physiology and Biophysics
Institute of Physiology, Pathohysiology and Biophysics, Unit of Physiology, Pathophysiology, and Experimental Endocrinology

Project(s): Mechanism of nucleotide-mediated inhibition of mitochondrial uncoupling proteins

UCP1 and UCP3 are members of the uncoupling protein (UCP) subfamily and are localized in the inner mitochondrial membrane. Whereas UCP1's central role in non-shivering thermogenesis is acknowledged, the function and even tissue expression pattern of UCP3 are still under dispute. Because UCP3 properties regarding transport of protons are qualitatively identical to those of UCP1, its expression in brown adipose tissue (BAT) alongside UCP1 requires justification. In this work, we tested whether any correlation exists between the expression of UCP1 and UCP3 in BAT by quantification of protein amounts in mouse tissues at physiological conditions, in cold-acclimated and UCP1 knockout mice. Quantification using recombinant UCP3 revealed that the UCP3 amount in BAT (0.51ng/(μg total tissue protein)) was nearly one order of magnitude higher than that in muscles and heart. Cold-acclimated mice showed an approximate three-fold increase in UCP3 abundance in BAT in comparison to mice in thermoneutral conditions. Surprisingly, we found a significant decrease of UCP3 in BAT of UCP1 knockout mice, whereas the protein amount in skeletal and heart muscles remained constant. UCP3 abundance decreased even more in cold-acclimated UCP1 knockout mice. Protein quantification in UCP3 knockout mice revealed no compensatory increase in UCP1 or UCP2 expression. Our results do not support the participation of UCP3 in thermogenesis in the absence of UCP1 in BAT, but clearly demonstrate the correlation in abundance between both proteins. The latter is important for understanding UCP3's function in BAT. Copyright © 2015 The Authors. Published by Elsevier B.V. All rights reserved.

Keywords Pubmed: Adipose Tissue, Brownmetabolism
Ion Channelsphysiology
Mice, Inbred C57BL
Mice, Knockout
Mitochondrial Proteinsphysiology
Uncoupling Protein 1
Uncoupling Protein 3

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