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Selected Publication:

Publication type: Journal Article
Document type: Full Paper

Year: 2016

Author(s): Rodríguez-Gómez, IM; Talker, SC; Käser, T; Stadler, M; Hammer, SE; Saalmüller, A; Gerner, W

Title: Expression of T-bet, Eomesodermin and GATA-3 in porcine αβ T cells.

Source: Dev Comp Immunol. 2016; 60:115-126

Authors Vetmeduni Vienna:

Gerner Wilhelm
Hammer Sabina
Käser Tobias
Rodriguez Gomez Irene Magdalena
Saalmüller Armin
Talker Stephanie

Vetmed Research Units
Institute of Immunology

The transcription factors GATA-3, T-bet and Eomesodermin play important roles in T-cell development, differentiation and memory formation. However, their expression has not been studied in great detail in porcine T cells. We report on protein expression at the single cell-level of these transcription factors in thymocytes and mature αβ T cells. GATA-3 expression was found in γδ(-) thymocytes, with decreasing expression from the CD4(-)CD8α(-) stage towards single-positive stages. Extra-thymic CD4(+) T cells but not CD8β(+) T cells expressed low levels of GATA-3, which decreased with age. CD4(+) and CD8β(+) T-bet(+) cells mainly displayed a CD8α(+)CD27(-) and perforin(+)CD27(dim/-) phenotype, respectively and had the capacity for IFN-γ production; indicative of an effector/effector memory phenotype. Eomesodermin(+) αβ T cells had mixed phenotypes in regard to CD8α, CD27 and perforin expression. In conclusion, our data so far support the hitherto reported roles for GATA-3 in T-cell development and T-bet for Th1 effector-differentiation, but question the role of Eomesodermin for memory formation of porcine T-cells.

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