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Type of publication: Journal Article
Type of document: Full Paper

Year: 2016

Authors: Awad, WA; Mann, E; Dzieciol, M; Hess, C; Schmitz-Esser, S; Wagner, M; Hess, M

Title: Age-Related Differences in the Luminal and Mucosa-Associated Gut Microbiome of Broiler Chickens and Shifts Associated with

Source: Front Cell Infect Microbiol. 2016; 6:154



Authors Vetmeduni Vienna:

Awad Wageha
Dzieciol Monika
Hess Claudia
Hess Michael
Schmitz-Esser Stephan
Selberherr Evelyne
Wagner Martin

Vetmed Research Units
University Clinic for Poultry and Fish Medicine, Clinical Unit of Poultry Medicine
Institute of Food Safety, Food Technology and Veterinary Public Health, Unit of Food Microbiology


Project(s): Etablierung einer universitären Kooperation und eines transnationalen "Centre of Excellence" für Geflügel


Abstract:
Despite the importance of gut microbiota for broiler performance and health little is known about the composition of this ecosystem, its development and response towards bacterial infections. Therefore, the current study was conducted to address the composition and structure of the microbial community in broiler chickens in a longitudinal study from day 1 to day 28 of age in the gut content and on the mucosa. Additionally, the consequences of a Campylobacter (C.) jejuni infection on the microbial community were assessed. The composition of the gut microbiota was analyzed with 16S rRNA gene targeted Illumina MiSeq sequencing. Sequencing of 130 samples yielded 51,825,306 quality-controlled sequences, which clustered into 8285 operational taxonomic units (OTUs; 0.03 distance level) representing 24 phyla. Firmicutes, Proteobacteria, Bacteroidetes, Actinobacteria, and Tenericutes were the main components of the gut microbiota, with Proteobacteria and Firmicutes being the most abundant phyla (between 95.0 and 99.7% of all sequences) at all gut sites. Microbial communities changed in an age-dependent manner. Whereas, young birds had more Proteobacteria, Firmicutes, and Tenericutes dominated in older birds (>14 days old). In addition, 28 day old birds had more diverse bacterial communities than young birds. Furthermore, numerous significant differences in microbial profiles between the mucosa and luminal content of the small and large intestine were detected, with some species being strongly associated with the mucosa whereas others remained within the luminal content of the gut. Following oral infection of 14 day old broiler chickens with 1 × 108 CFU of C. jejuni NCTC 12744, it was found that C. jejuni heavily colonized throughout the small and large intestine. Moreover, C. jejuni colonization was associated with an alteration of the gut microbiota with infected birds having a significantly lower abundance of Escherichia (E.) coli at different gut sites. On the contrary, the level of Clostridium spp. was higher in infected birds compared with birds from the negative controls. In conclusion, the obtained results demonstrate how the bacterial microbiome composition changed within the early life of broiler chickens in the gut lumen and on the mucosal surface. Furthermore, our findings confirmed that the Campylobacter carrier state in chicken is characterized by multiple changes in the intestinal ecology within the host.

Keywords Pubmed: Age Factors
Animals
Campylobacter Infectionsmicrobiologyveterinary
Chickens
Cluster Analysis
DNA, Bacterialchemistrygenetics
DNA, Ribosomalchemistrygenetics
Gastrointestinal Microbiome
Gastrointestinal Tractmicrobiology
Intestinal Mucosamicrobiology
Longitudinal Studies
Phylogeny
RNA, Ribosomal, 16Sgenetics
Sequence Analysis, DNA

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