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Type of publication: Journal Article
Type of document: Full Paper

Year: 2018

Authors: Ferraz, MAMM; Rho, HS; Hemerich, D; Henning, HHW; van Tol, HTA; Hölker, M; Besenfelder, U; Mokry, M; Vos, PLAM; Stout, TAE; Le Gac, S; Gadella, BM

Title: An oviduct-on-a-chip provides an enhanced in vitro environment for zygote genome reprogramming.

Source: Nat Commun. 2018; 9(1):4934



Authors Vetmeduni Vienna:

Besenfelder Urban

Vetmed Research Units
Institute of Animal Breeding and Genetics, Unit of Reproductive Biology


Abstract:
Worldwide over 5 million children have been conceived using assisted reproductive technology, and research has concentrated on increasing the likelihood of ongoing pregnancy. However, studies using animal models have indicated undesirable effects of in vitro embryo culture on offspring development and health. In vivo, the oviduct hosts a period in which the early embryo undergoes complete reprogramming of its (epi)genome in preparation for the reacquisition of (epi)genetic marks. We designed an oviduct-on-a-chip platform to better investigate the mechanisms related to (epi)genetic reprogramming and the degree to which they differ between in vitro and in vivo embryos. The device supports more physiological (in vivo-like) zygote genetic reprogramming than conventional IVF. This approach will be instrumental in identifying and investigating factors critical to fertilization and pre-implantation development, which could improve the quality and (epi)genetic integrity of IVF zygotes with likely relevance for early embryonic and later fetal development.

Keywords Pubmed: Animals
Cattle
Cells, Cultured
Cellular Reprogramminggenetics
Epigenesis, Genetic
Female
Fertilization in Vitroinstrumentationmethods
Gene Expression Profiling
Gene Ontology
Genomicsmethods
Humans
Oviductscytologymetabolism
Pregnancy
Zygotegrowth & developmentmetabolism

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