University of Veterinary Medicine Vienna - Research portal

Selected Publication:

Open Access Logo

Type of publication: Journal Article
Type of document: Full Paper

Year: 2019

Authors: Martini, F; Eckmair, B; Štefanić, S; Jin, C; Garg, M; Yan, S; Jiménez-Castells, C; Hykollari, A; Neupert, C; Venco, L; Varón Silva, D; Wilson, IBH; Paschinger, K

Title: Highly modified and immunoactive N-glycans of the canine heartworm.

Source: Nat Commun. 2019; 10(1):75

Authors Vetmeduni Vienna:

Yan Shi

Vetmed Research Units
Institute of Parasitology

Project(s): Biosynthesis of helminth N-glycoproteins in insect cells

The canine heartworm (Dirofilaria immitis) is a mosquito-borne parasitic nematode whose range is extending due to climate change. In a four-dimensional analysis involving HPLC, MALDI-TOF-MS and MS/MS in combination with chemical and enzymatic digestions, we here reveal an N-glycome of unprecedented complexity. We detect N-glycans of up to 7000 Da, which contain long fucosylated HexNAc-based repeats, as well as glucuronylated structures. While some modifications including LacdiNAc, chitobiose, α1,3-fucose and phosphorylcholine are familiar, anionic N-glycans have previously not been reported in nematodes. Glycan array data show that the neutral glycans are preferentially recognised by IgM in dog sera or by mannose binding lectin when antennal fucose and phosphorylcholine residues are removed; this pattern of reactivity is reversed for mammalian C-reactive protein, which can in turn be bound by the complement component C1q. Thereby, the N-glycans of D. immitis contain features which may either mediate immunomodulation of the host or confer the ability to avoid immune surveillance.

© University of Veterinary Medicine ViennaHelp and Downloads