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Selected Publication:

Type of publication: Doctoral Thesis
Type of document:

Year: 2003

Authors: Suchodolski, J

Title: Canine Pepsinogen: Purification and Partial Characterization, Development and Validation of an Enzyme-linked Immunosorbent Assay for Measurement of Pepsinogen in Serum, and Clinical Application.

Source: Dissertation, Vet. Med. Univ. Wien, pp. 89.

Advisor(s):

Thalhammer Johann


Abstract:
The aim of this study was to purify different isoforms of canine pepsinogen (cPG) in order to develop and validate an ELISA for measurement of cPG, and to evaluate the clinical usefulness of serum cPG concentration as a marker for gastritis in dogs. A mucosal extract was prepared from dog stomach and further purified by use of hydroxyapatite, size-exclusion, and anion-exchange chromatography. An ELISA for cPG A was developed and validated, a reference range established, and postprandial alterations in serum PG A were evaluated. Finally, serum cPG was evaluated in 19 dogs with gastritis. Two immunological different groups of canine PG were identified: PG A and PG B. Groups of isoforms differed in their molecular weights (MW) and isoelectric points (pI). PG B appeared to be a dimer with a MW of approximately 34,100 and a pI of 4.9. PG A separated into several isoforms. The MW for the various isoforms of PG A ranged from 34,200 to 42,100 and their pI ranged from 4.0 to ; 3.0. The N-terminal amino acid sequence for the first 25 amino acid residues for cPG A and B showed good homology with the amino acid sequence for these proteins in other species. Only secretory cells in the gastric cardia and fundus showed immunoreactivity for cPG A. The ELISA for measuring canine pepsinogen A in canine serum was sufficiently sensitive, linear, accurate, precise, and reproducible. Serum cPG A increased significantly after feeding (p ; 0.001). Mean serum cPG A concentration was significantly higher in dogs with gastritis than in clinically healthy dogs (p = 0.012). However, only 21 % of dogs with gastritis had serum cPG A concentrations above the upper limit of the reference range, indicating that cPG A is a relatively insensitive marker for canine gastritis.

Keywords:
pepsinogen A / pepsinogen B / dog / ELISA / immunolocalization / feeding / gastritis


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