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Publication type: Baccalaureate Thesis

Year: 2011

Author(s): Geyer, Antonia

Title: Equines Herpesvirus Typ 2: Etablierung einer Realtime-PCR zur Anwendung in der Routinediagnostik.

Other title: Equine Herpesvirus Type 2: Establishment of a Real-Time PCR for Application in Routine Diagnostics

Source: Bakkalaureatsarbeit, Vet. Med. Univ. Wien, pp. 30.


Authors Vetmeduni Vienna:

Geyer Antonia

Advisor(s):
Möstl Karin

Reviewer(s):
Klein Dieter

Vetmed Research Units:
Institute of Virology


Graduation date: 09.09.11


Abstract:
The diagnosis of EHV-2 is a common issue in the routine diagnostics of eye diseases of horses. For that reason, this study presents a design of a Realtime-PCR for the EHV-2 routine diagnostics and first investigations about sensitivity and specificity. The Maximal Identity of the amplification product of the EHV-2 strain LK was 92%, compared to the sequence published in the database (EHV-2 complete genome, Acc.Nr. U20824). The presented combination of primer and probes was able to detect nucleic acids of EHV-2 in field samples, taken from diseased eyes of horses. Additionally the primer/probe-combination was also able to distinguish between EHV-2 and EHV-5, which are closely related. In one case, the detection of the DNA of asinine herpesvirus 5 (AHV-5) could not be excluded. Compared to the cell culture, the designed Realtime-PCR was able to detect nucleic acid up to a dilution by the factor of 100,000 (according to 0.00001 TCID50). These results indicate a high sensitivity of the established Realtime-PCR.


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