The diagnosis of EHV-2 is a common issue in the routine diagnostics of eye
diseases of horses. For that reason, this study presents a design of a Realtime-PCR
for the EHV-2 routine diagnostics and first investigations about sensitivity and
The Maximal Identity of the amplification product of the EHV-2 strain LK was 92%,
compared to the sequence published in the database (EHV-2 complete genome,
Acc.Nr. U20824). The presented combination of primer and probes was able to
detect nucleic acids of EHV-2 in field samples, taken from diseased eyes of horses.
Additionally the primer/probe-combination was also able to distinguish between
EHV-2 and EHV-5, which are closely related. In one case, the detection of the DNA
of asinine herpesvirus 5 (AHV-5) could not be excluded.
Compared to the cell culture, the designed Realtime-PCR was able to detect nucleic
acid up to a dilution by the factor of 100,000 (according to 0.00001 TCID50). These
results indicate a high sensitivity of the established Realtime-PCR.