Minimally invasive surgery has gained popularity in veterinary surgery because of theadvantages of faster recovery and reduced postoperative pain, as known from humanmedicine. Laparoscopic sterilization has become a common alternative to open surgery indogs and is being performed with different techniques. Laparoscopic ovariohysterectomy witha modified single-port glove-port-technique has not been evaluated so far in veterinarymedicine. For this study, forty-two healthy dogs of different breeds, aged between 6 monthsand 81 months and with a bodyweight of 5 kg to 39.5 kg were included in this study.Surgical times, from assembly of the glove-port to skin closure and total duration of surgery,from first incision to skin closure was recorded and compared with previously publishedstudies on laparoscopic ovariohysterectomy in dogs. Intraoperative and postoperativecomplications were registered.The single-port laparoscopic ovariohysterectomy with a modified glove-port-technique wasfaster than previously reported techniques. No major intraoperative complications occurredbut postoperative local wound complications consisting of local inflammation and wounddehiscence occurred in 29% of dogs. The influence of the pressure of the plastic ring of thewound retractor on the skin margins might be an explanation but warrants additionalinvestigation.In conclusion the modified glove-port technique consisting of a surgical glove, an Alexiswound retractor and commercially available trocars represent a cost-effective alternative toconventional single-port systems. This technique represents a valuable add-on to currentsingle-port laparoscopic surgeries in veterinary medicine.