Introduction: Advanced bipolar vessel sealing devices are widely used in human and veterinarymedicine to reduce the operation time and intraoperative blood loss. Because most devices aremade for a single use, their application is cost-intensive. The aim of this study was to investigate theeffects of multiple uses and sterilization on the performance of bipolar vessel sealing devices. Ourhypothesis was that the LigaSure and Caiman devices, which are intended for single use only, wouldshow a decreasing ability to perform safe seals over multiple operations and sterilizations comparedto that of the reuseable MarSeal.Methods: The burst pressures of sealed porcine renal arteries were compared between thedisposable devices „LigaSure“ (Valleylab) and „Caiman“ (Braun Vetcare) and the reuseable device„MarSeal“ (KLS Martin). Additionally, impact of the sterilization process was investigated, as well asthe sealing time, number of cutting activations and tissue adhesion were noted.Results: The disposable devices showed reliable performances over multiple activations andsterilization cycles, except for a single Caiman device. Seals created with all devices achieved supraphysiologicburst pressures, with the highest pressure measured in a cycle using a MarSeal device.Discussion / Conclusion: During 25 activations with and without intermittent sterilization, nocorrelation was found between increasing seal numbers and decreasing burst pressure. However, thenumber of cycles in our study was limited to five. For limited numbers of cycles and sterilizationprocedures, seals created with the disposable vessel sealing devices LigaSure and Caiman achievedburst pressures comparable to those produced with the reuseable MarSeal.