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Selected Publication:

Publication type: Journal Article
Document type: Full Paper

Year: 2008

Author(s): Böhm, C; Cichna-Markl, M; Brenn-Struckhofova, Z; Razzazi-Fazeli, E

Title: Development of a selective sample clean-up method based on immuno-ultrafiltration for the determination of deoxynivalenol in maize.

Source: J Chromatogr A. 2008; 1202(2):111-117



Authors Vetmeduni Vienna:

Razzazi-Fazeli Ebrahim

Vetmed Research Units
Institute of Animal Nutrition and Functional Plant Compounds
VetCore


Abstract:
This paper describes the development of a highly selective analytical method for the determination of deoxynivalenol (DON) in maize. The developed method is based on immuno-ultrafiltration (IUF) and is the first application of IUF as a clean-up strategy in food analysis. Quantification of DON was carried out by high-performance liquid chromatography with ultraviolet detection. In contrast to immunoaffinity chromatography, in IUF the antibodies are not bound to a solid support material but used in free form, thus making it possible to avoid the critical immobilisation step. Sample clean-up by IUF proved to be as selective as clean-up using commercially available immunoaffinity columns. The limit of detection (S/N=3) of the analytical method was found to be 74 ng DON/g maize. Repeated analysis of a certified maize reference material on four different days resulted in a mean recovery of 93% with a standard deviation of 10%.

Keywords Pubmed: Chromatography, Affinity
Chromatography, High Pressure Liquid
Food Analysis/methods*
Mycotoxins/analysis
Reproducibility of Results
Trichothecenes/analysis*
Ultrafiltration/methods
Zea mays/chemistry*


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