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Publication type: Journal Article
Document type: Full Paper

Year: 2008

Author(s): Mader, E; Lukas, B; Novak, J

Title: A strategy to setup codominant microsatellite analysis for high-resolution-melting-curve-analysis (HRM).

Source: BMC Genet. 2008; 9:69



Authors Vetmeduni Vienna:

Lukas Brigitte
Mader Eduard
Novak Johannes

Vetmed Research Units
Institute of Animal Nutrition and Functional Plant Compounds


Project(s): Molecular Phylogeny and Phytochemistry of the Genus Origanum


Abstract:
High resolution melting curve analysis (HRM) is a technique that measures exactly the decreasing fluorescence of intercalating dye in the process of dissociation of double stranded DNA. The measurement is immediately following PCR in a one-step, closed-tube method. The shape of the melting curve depends on the GC content, length and sequence of the amplicon. Hence it is a powerful, fast and cheap method to detect Single Nucleotide Polymorphisms (SNPs) and other mutations.

Keywords Pubmed: Alleles
DNA/genetics
Microsatellite Repeats/genetics*
Minisatellite Repeats
Nucleic Acid Denaturation
Origanum/genetics
Polymerase Chain Reaction/methods
Polymorphism, Single Nucleotide
Sequence Analysis, DNA/methods*
Temperature*


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