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Publication type: Journal Article
Document type: Full Paper

Year: 2004

Author(s): Grassi, P; Novak, J; Steinlesberger, H; Franz, C

Title: A direct liquid, non-equilibrium solid-phase micro-extraction application for analysing chemical variation of single peltate trichomes on leaves of Salvia officinalis.

Source: Phytochem Anal. 2004; 15(3):198-203



Authors Vetmeduni Vienna:

Franz Chlodwig
Grassi Paolo
Novak Johannes

Vetmed Research Units
Institute of Animal Nutrition and Functional Plant Compounds


Project(s): Investigation of Biochemical and Genetical Diversity of Terpenoid Biosynthesis for the Production of High Value-Added Compounds


Abstract:
A non-equilibrium solid phase micro-extraction application was tested for the rapid extraction of essential oil from single oil glands of sage and the oil components compared with those determined by solvent extract and distillation. Oil glands were directly ruptured with a polydimethylsiloxane-coated fused silica fibre and the essential oil was sorbed. Three insertion levels of an individual plant of Salvia officinalis involving an immature apical young leaf, an expanding and a fully developed leaf, respectively, were used to determine the applicability of this method. Thirty-eight components in the oil could be identified by GC-MS. The method only showed small semi-quantitative differences compared with conventional methods. Chemical variation of single oil glands within the immature and premature leaf was higher than within the homogeneous mature leaf. The intermediary, still-expanding leaf was used to carry out a detailed study of the glands. The basal region of the intermediary leaf contained compounds in high conformity with the young leaf trichomes. The remaining oil glands of this leaf showed inconsistent accumulation patterns.

Keywords Pubmed: Chromatography, High Pressure Liquid
Microchemistry
Plant Leaves/chemistry
Plant Oils/analysis*
Principal Component Analysis
Salvia officinalis/chemistry*
Sesquiterpenes/analysis*


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