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Publication type: Journal Article
Document type: Full Paper

Year: 2012

Author(s): Compaoré, M; Lamien, CE; Lamien-Meda, A; Vlase, L; Kiendrebeogo, M; Ionescu, C; Nacoulma, OG

Title: Antioxidant, xanthine oxidase and lipoxygenase inhibitory activities and phenolics of Bauhinia rufescens Lam. (Caesalpiniaceae).

Source: Nat Prod Res. 2012; 26(11):1069-1074



Authors Vetmeduni Vienna:

Lamien-Meda Aline

Vetmed Research Units
Institute of Animal Nutrition and Functional Plant Compounds


Abstract:
An aqueous acetone extract of the stem with the leaves of Bauhinia rufescens and its fractions were analysed for their antioxidant and enzyme-inhibitory activities, as well as their phytochemical composition. For measurement of the antioxidant activities, the 2,2-diphenyl-1-picrylhydrazyl, 2,2"-azinobis(3-ethylbenzoline-6-sulphonate) and the ferric-reducing methods were used. The results indicated that the aqueous acetone, its ethyl acetate and n-butanol fractions possessed considerable antioxidant activity. Further, the xanthine oxidase and lipoxygenase inhibitory assays showed that the n-butanol fraction possessed compounds that can inhibit both these enzymes. In the phytochemical analysis, the ethyl acetate and the n-butanol fractions of the aqueous acetone extract were screened by HPLC-MS for their phenolic content. The results indicated the presence of hyperoside, isoquercitrin, rutin quercetin, quercitrin, p-coumaric and ferulic acids in the non-hydrolysed fractions. In the hydrolysed fractions, kaempferol, p-coumaric and ferulic acids were identified.

Keywords Pubmed: Antioxidants/pharmacology*
Bauhinia/chemistry*
Chromatography, High Pressure Liquid
Enzyme Inhibitors/pharmacology*
Lipoxygenase Inhibitors/pharmacology*
Mass Spectrometry
Phenols/pharmacology*
Xanthine Oxidase/antagonists & inhibitors*


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