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Type of publication: Journal Article
Type of document: Full Paper

Year: 2013

Authors: Valencak, TG; Ruf, T

Title: Phospholipid composition and longevity: lessons from Ames dwarf mice.

Source: Age (Dordr). 2013; 35(6):2303-2313



Authors Vetmeduni Vienna:

Ruf Thomas
Valencak Teresa

Vetmed Research Units
Research Institute of Wildlife Ecology


Project(s): Membrane fatty acid composition in AMes dwarf mice


Abstract:
Membrane fatty acid (FA) composition is correlated with longevity in mammals. The "membrane pacemaker hypothesis of ageing" proposes that animals which cellular membranes contain high amounts of polyunsaturated FAs (PUFAs) have shorter life spans because their membranes are more susceptible to peroxidation and further oxidative damage. It remains to be shown, however, that long-lived phenotypes such as the Ames dwarf mouse have membranes containing fewer PUFAs and thus being less prone to peroxidation, as would be predicted from the membrane pacemaker hypothesis of ageing. Here, we show that across four different tissues, i.e., muscle, heart, liver and brain as well as in liver mitochondria, Ames dwarf mice possess membrane phospholipids containing between 30 and 60 % PUFAs (depending on the tissue), which is similar to PUFA contents of their normal-sized, short-lived siblings. However, we found that that Ames dwarf mice membrane phospholipids were significantly poorer in n-3 PUFAs. While lack of a difference in PUFA contents is contradicting the membrane pacemaker hypothesis, the lower n-3 PUFAs content in the long-lived mice provides some support for the membrane pacemaker hypothesis of ageing, as n-3 PUFAs comprise those FAs being blamed most for causing oxidative damage. By comparing tissue composition between 1-, 2- and 6-month-old mice in both phenotypes, we found that membranes differed both in quantity of PUFAs and in the prevalence of certain PUFAs. In sum, membrane composition in the Ames dwarf mouse supports the concept that tissue FA composition is related to longevity.

Keywords Pubmed: Animals
Brain/growth & development
Brain/metabolism
Brain/pathology
Cell Membrane/metabolism
Cells, Cultured
Disease Models, Animal
Dwarfism/metabolism*
Fatty Acids/metabolism*
Female
Lipid Peroxidation
Longevity/physiology*
Male
Mice
Mitochondria, Liver/metabolism
Mitochondria, Liver/pathology
Oxidative Stress*
Phospholipids/metabolism*


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