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Type of publication: Journal Article
Type of document: Full Paper

Year: 2013

Authors: Loncaric, I; Stalder, GL; Mehinagic, K; Rosengarten, R; Hoelzl, F; Knauer, F; Walzer, C

Title: Comparison of ESBL--and AmpC producing Enterobacteriaceae and methicillin-resistant Staphylococcus aureus (MRSA) isolated from migratory and resident population of rooks (Corvus frugilegus) in Austria.

Source: PLoS One. 2013; 8(12):e84048

Authors Vetmeduni Vienna:

Hölzl Franz
Knauer Felix
Loncaric Igor
Rosengarten Renate
Stalder Gabrielle
Walzer Christian

Vetmed Research Units
Institute of Microbiology
Research Institute of Wildlife Ecology

In order to test whether rooks (Corvus frugilegus) represent good indicators for the potential circulation of antibiotics in their native habitat, two populations with different migratory behavior were tested for the presence of beta-lactamase producing Enterobacteriaceae and methicillin-resistant Staphylococcus aureus (MRSA). In all, 54 and 102 samples of fresh feces of a migratory and a resident population were investigated. A total of 24 and 3 cefotaxime-resistant enterobacterial isolates were obtained from the migratory and resident population, respectively. In these isolates CTX-M-1 (n = 15), CTX-M-3 (n = 3), and CTX-M-15 (n = 3) genes were detected. TEM-1 and OXA-1 were associated with CTX-M in 3 and 2 isolates, respectively. In two E. coli isolates CMY-2 could be detected, where from one isolate displayed an overexpression of chromosomal AmpC as well. Among E. coli isolates the most common phylogenetic group was A (n = 11) and ST1683 (n = 5). In one E. coli of B2-ST131 the rfbO25b locus was detected. Three Enterobacter isolates were stably derepressed AmpC-producers. In five samples of the migratory population, PVL positive MRSA could be isolated. Two isolates were typed SCCmec IVa, spa type t127, and ST1. Three isolates carried a SCCmec type IVc, with spa type t852 and ST22. The highly significant difference of the occurrence of antibiotic resistance between the migratory population from eastern Europe compared to resident population in our study indicates that rooks may be good indicator species for the evaluation of environmental contamination with antibiotic resistant bacteria, especially due to their ecology, foraging behavior and differing migratory behavior.

Keywords Pubmed: Ampicillin/metabolism*
Animal Diseases/microbiology*
Animal Migration*
Anti-Bacterial Agents/pharmacology
DNA, Bacterial/genetics
Drug Resistance, Microbial/genetics
Enterobacteriaceae/isolation & purification*
Enterobacteriaceae Infections/drug therapy
Enterobacteriaceae Infections/microbiology
Enterobacteriaceae Infections/veterinary
Escherichia coli/classification
Escherichia coli/genetics
Escherichia coli/isolation & purification*
Escherichia coli Infections/drug therapy
Escherichia coli Infections/microbiology
Escherichia coli Infections/veterinary*
Methicillin-Resistant Staphylococcus aureus/genetics
Methicillin-Resistant Staphylococcus aureus/isolation & purification*
Microbial Sensitivity Tests
Polymerase Chain Reaction
Staphylococcal Infections/drug therapy
Staphylococcal Infections/microbiology
Staphylococcal Infections/veterinary

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