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Selected Publication:

Type of publication: Journal Article
Type of document: Full Paper

Year: 2014

Authors: Gottstein, B; Frey, CF; Campbell-Palmer, R; Pizzi, R; Barlow, A; Hentrich, B; Posautz, A; Ryser-Degiorgis, MP

Title: Immunoblotting for the serodiagnosis of alveolar echinococcosis in alive and dead Eurasian beavers (Castor fiber).

Source: Vet Parasitol. 2014; 205(1-2):113-118



Authors Vetmeduni Vienna:

Posautz Annika

Vetmed Research Units
Research Institute of Wildlife Ecology


Abstract:
A novel species-specific anti-beaver-IgG-alkaline-phosphatase conjugate was synthesized for the development of a new serological test for echinococcosis in beavers. Two different ELISAs conventionally used for human Echinococcus multilocularis serology (Em18-ELISA and Em2-ELISA) yielded diagnostic sensitivities of 0% and 46%, respectively. In contrast, the subsequently developed immunoblotting assay gave an 85% diagnostic sensitivity (11 out of 13 beavers with alveolar echinococcosis were immunoblotting-positive, i.e. showed reactivity with a specific 21 Mr band), and maximal specificity. In conclusion, this immunoblotting assay should be the method of choice for use in serological studies on E. multilocularis in Eurasian beavers, and the test proved suitable to investigate both animals alive and post-mortem.

Keywords Pubmed: Animals
Antibodies, Helminth/blood*
Echinococcosis, Hepatic/diagnosis
Echinococcosis, Hepatic/veterinary*
Echinococcus/immunology
Immunoblotting/veterinary*
Rodentia/parasitology*
Sensitivity and Specificity


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