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Selected Publication:

Publication type: Journal Article
Document type: Full Paper

Year: 1998

Author(s): Razzazi-Fazeli, E; Schmid, RW

Title: Analytical specificity and optimization of particle-beam measurements for some biopharmaceutical applications using a Hildebrand nebulizer.

Source: Rapid Commun Mass Sp (12), 23 1859-1866.



Authors Vetmeduni Vienna:

Razzazi-Fazeli Ebrahim

Vetmed Research Units
Institute of Animal Nutrition and Functional Plant Compounds


Abstract:
The present study shows how specific and selective particle-beam liquid chromatography mass spectrometry (PB-LC/MS) can be for certain biopharmaceutical applications and by which strategy maximum sensitivity can be achieved. The utility of PB-LC/MS in the analysis of different pharmaceutical drugs and endogenic substances with different molecular structures has been investigated. Using the PB interface in combination with a triple quadrupole mass spectrometer, the effects of mobile phase composition and flow rate, interface parameters-such as desolvation temperature and pressure of the nebulization gas-and the temperature of the ion source, on detection limits and sensitivity, are demonstrated, A particular substance specificity of this interface type for creatinine, naloxone and methadone could be observed after optimizing the transmission of the interface, The best sensitivity is achieved only in a narrow working range, indicating the necessity of an individual optimization of interface conditions for each substance. Linearity, sensitivity and precision of transfer rate using the EI and selected-ion monitoring modes were demonstrated. Investigation of the so-called "carrier effect" on the transfer of the selected compounds indicated that the addition of ammonium acetate as "carrier" changed the detection sensitivity in a completely different order. (C) 1998 John Wiley & Sons, Ltd.


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