Pommer, E; Zitterl-Eglseer, K; Lischka, B; Kubesch, K; Van den Hoven, R
A sensitive method to study pharmacokinetics and bioavailability of Petasites hybridus leaf extract Ze 339 in horses following oral administration of different formulations - a pilot study.
LC/MS/MS zur Studie von Pharmakokinetik und Bioverfügbarkeit von Petasites hybridus Blatt Extrakt Ze 339 bei Pferden nach oraler Gabe unterschiedlicher Darreichungsformen – eine Pilot-Studie
Wien Tierarztl Monat. 2017; 104(11-12): 353-361.
Orally administered extracts of butterbur are a popular treatment option for equine asthma syndrome in veterinary practice, despite the fact that none of these preparations are registered as phytopharmaceutical products for horses. The aim of the present study was to establish a sensitive LC/MS/MS method for measurement of petasin, isopetasin and neopetasin in plasma and urine as markers for the active constituents of a Petasites hybridus preparation. Four horses were dosed via a naso-gastric tube with 10 or 40 mg/kg bm Petasites hybridus dry extract in watery or oily suspension. Comparing the watery suspension with the oily one, the watery suspension showed higher plasma levels of petasin, isopetasin and neopetasin, although drug absorption was much faster with the oily suspension. Petasin was better absorbed than isopetasin; and isopetasin better absorbed than neopetasin. The administration of 10 mg piperine as absorption enhancer did not result in higher plasma levels. For petasin the C-max varied between 19 and 673 pg/ml, t(max) varied between 0.5 and 6 h and the AUCs varied from 100 to 2733 pg/ml*h. The established. LC/MS/MS method is very sensitive for petasins and suitable for analysis of plasma and urine samples to a limit of detection of 10 pg/ml.