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Type of publication: Journal Article
Type of document: Full Paper

Year: 2020

Authors: Kreiter, J; Beitz, E; Pohl, EE

Title: A Fluorescence-Based Method to Measure ADP/ATP Exchange of Recombinant Adenine Nucleotide Translocase in Liposomes.

Source: Biomolecules. 2020; 10(5):685



Authors Vetmeduni Vienna:

Kreiter J├╝rgen
Pohl Elena

Vetmed Research Units
Institute of Physiology, Pathohysiology and Biophysics, Unit of Physiology and Biophysics


Project(s): Proton leak through Adenine Nucleotide Translocase


Abstract:
Several mitochondrial proteins, such as adenine nucleotide translocase (ANT), aspartate/glutamate carrier, dicarboxylate carrier, and uncoupling proteins 2 and 3, are suggested to have dual transport functions. While the transport of charge (protons and anions) is characterized by an alteration in membrane conductance, investigating substrate transport is challenging. Currently, mainly radioactively labeled substrates are used, which are very expensive and require stringent precautions during their preparation and use. We present and evaluate a fluorescence-based method using Magnesium Green (MgGr


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