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Type of publication: Journal Article
Type of document: Full Paper

Year: 2021

Authors: Grünzweil, OM; Palmer, L; Cabal, A; Szostak, MP; Ruppitsch, W; Kornschober, C; Korus, M; Misic, D; Bernreiter-Hofer, T; Korath, ADJ; Feßler, AT; Allerberger, F; Schwarz, S; Spergser, J; Müller, E; Braun, SD; Monecke, S; Ehricht, R; Walzer, C; Smodlaka, H; Loncaric, I

Title: Presence of β-Lactamase-producing Enterobacterales and Salmonella Isolates in Marine Mammals.

Source: Int J Mol Sci. 2021; 22(11):5905

Authors Vetmeduni Vienna:

Bernreiter-Hofer Tanja
Korath Anna
Loncaric Igor
Spergser Joachim
Szostak Michael
Walzer Christian

Vetmed Research Units
Research Institute of Wildlife Ecology, Conservation Medicine
Institute of Microbiology
University Clinic for Swine

Marine mammals have been described as sentinels of the health of marine ecosystems. Therefore, the aim of this study was to investigate (i) the presence of extended-spectrum β-lactamase (ESBL)- and AmpC-producing Enterobacterales, which comprise several bacterial families important to the healthcare sector, as well as (ii) the presence of Salmonella in these coastal animals. The antimicrobial resistance pheno- and genotypes, as well as biocide susceptibility of Enterobacterales isolated from stranded marine mammals, were determined prior to their rehabilitation. All E. coli isolates (n = 27) were screened for virulence genes via DNA-based microarray, and twelve selected E. coli isolates were analyzed by whole-genome sequencing. Seventy-one percent of the Enterobacterales isolates exhibited a multidrug-resistant (MDR) pheno- and genotype. The gene blaCMY (n = 51) was the predominant β-lactamase gene. In addition, blaTEM-1 (n = 38), blaSHV-33 (n = 8), blaCTX-M-15 (n = 7), blaOXA-1 (n = 7), blaSHV-11 (n = 3), and blaDHA-1 (n = 2) were detected. The most prevalent non-β-lactamase genes were sul2 (n = 38), strA (n = 34), strB (n = 34), and tet(A) (n = 34). Escherichia coli isolates belonging to the pandemic sequence types (STs) ST38, ST167, and ST648 were identified. Among Salmonella isolates (n = 18), S. Havana was the most prevalent serotype. The present study revealed a high prevalence of MDR bacteria and the presence of pandemic high-risk clones, both of which are indicators of anthropogenic antimicrobial pollution, in marine mammals.

Keywords Pubmed: Animals
Anti-Bacterial Agentspharmacology
Aquatic Organismsmicrobiology
Bacterial Proteinsgenetics
Bacterial Typing Techniques
Drug Resistance, Bacterial
Enterobacterdrug effectsenzymologygeneticsisolation & purification
Microbial Sensitivity Tests
Salmonelladrug effectsenzymologygeneticsisolation & purification
Virulence Factorsgenetics

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