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Type of publication: Journal Article
Type of document: Full Paper

Year: 2021

Authors: Huber, N; Mahr, K; Tóth, Z; Szarka, EZ; Çınar, YU; Salmón, P; Lendvai, ÁZ

Title: "The stressed bird in the hand": Influence of sampling design on the physiological stress response in a free-living songbird.

Source: Physiol Behav. 2021; 238:113488



Authors Vetmeduni Vienna:

Huber Nikolaus
Mahr Katharina

Vetmed Research Units
Konrad Lorenz Institute of Ethology, Unit of Ornithology
Institute of Food Safety, Food Technology and Veterinary Public Health, Unit of Veterinary Public Health and Epidemiology


Project(s): The role of IGF-1 in reproductive life history


Abstract:
Despite the widely used application of standardized capture-handling protocols to collect blood and assess the physiological stress response, the actual sampling design (e.g., timing and the number of blood samples) often differs between studies, and the potential implications for the measured physiological endpoints remain understudied. We, therefore experimentally tested the effects of repeated handling and multiple blood sampling on the stress response in wintering free-living great tits (Parus major). We modified a well-established sampling protocol of avian studies by adding either an additional blood sample or a "sham-manipulation" (i.e., handling associated with the blood sampling procedure without venepuncture), to disentangle the effects of handling stress and blood loss. We combined three different stress metrics along the endocrine-immune interface to investigate the acute short-term stress response: total corticosterone concentrations (Cort), the heterophil/lymphocyte ratio (H:L), and the Leucocyte Coping Capacity (LCC). Our study provided three key results: i) no relationship between Cort levels, LCC and H:L, confirming that these three parameters represent different physiological endpoints within the stress response; ii) contrasting dynamics in response to stress by the measured parameters and iii) no difference in physiological stress levels 30 min after capture due to one additional blood sampling or handling event. By optimising the sampling design, our results provide implications for animal welfare and planning experimental procedures on stress physiology in passerine species.Copyright © 2021 The Author(s). Published by Elsevier Inc. All rights reserved.


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