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Publication type: Journal Article
Document type: Full Paper

Year: 2005

Author(s): Nuryono, N; Noviandi, CT; Böhm, J; Razzazi-Fazeli, E

Title: A limited survey of zearalenone in Indonesian maize-based food and feed by ELISA and high performance liquid chromatography.

Source: Food Control (16), 1 65-71.

Authors Vetmeduni Vienna:

Böhm Josef
Razzazi-Fazeli Ebrahim

Vetmed Research Units
Institute of Animal Nutrition and Functional Plant Compounds

Project(s): Survey of Fusarium mycotoxins, Fumonisins and Zearalenone in corn and corn based products in Indonesia

A survey of zearalenone (ZON) in Indonesian maize-based food and feed was performed in 89 samples using an enzyme-linked immunosorbent assay (ELISA). Results indicated that 32 samples (36.0%) were contaminated in a range from 5.5 to 526 mug/kg. Only one highly contaminated sample was observed in a category of home-made food samples (>500 mug/kg) and the highest percentage of contaminated samples (>85.7%) was noted in a category of poultry feed. The positive samples based on the ELISA analyses were then confirmed using a high performance liquid chromatography (HPLC) method in combination with an immuno-affinity column for clean-up. Prior to sample measurement, the HPLC method was validated using a blank maize matrix. The overall average recovery of blank maize spiked with ZON at levels of 200-750 mug/kg were 100.2%. Detection limit based on a signal-to-noise ratio 3:1 was 3.0 mug/kg for ZON in maize samples and the linear range applicable for analyses was 5-900 mug/kg of ZON. Of 32 samples confirmed by HPLC, 25 samples were observed to be contaminated in a comparable range from 6.9 to 589 mug/kg. Comparative analysis of 25 positive samples using the ELISA and HPLC methods showed a reasonable correlation (R-2 = 0.9258). (C) 2004 Elsevier Ltd. All rights reserved.

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