University of Veterinary Medicine Vienna - Research portal

Diagrammed Link to Homepage University of Veterinary Medicine, Vienna

Selected Publication:

Open Access Logo

Type of publication: Journal Article
Type of document: Review Article

Year: 2021

Authors: Ramsauer, AS; Wachoski-Dark, GL; Fraefel, C; Ackermann, M; Brandt, S; Grest, P; Knight, CG; Favrot, C; Tobler, K

Title: Establishment of a three-dimensional in vitro model of equine papillomavirus type 2 infection.

Source: Viruses. 2021; 13(7):1404



Authors Vetmeduni Vienna:

Brandt Sabine
Ramsauer Anna Sophie

Vetmed Research Units
University Equine Clinic, Clinical Unit of Equine Internal Medicine
University Equine Clinic, Clinical Unit of Equine Surgery


Abstract:
There is growing evidence that equine papillomavirus type 2 (EcPV2) infection is etiologically associated with the development of genital squamous cell carcinoma (SCC) and precursor lesions in equids. However, the precise mechanisms underlying neoplastic progression remain unknown. To allow the study of EcPV2-induced carcinogenesis, we aimed to establish a primary equine cell culture model of EcPV2 infection. Three-dimensional (3D) raft cultures were generated from equine penile perilesional skin, plaques and SCCs. Using histological, molecular biological and immunohistochemical methods, rafts versus corresponding natural tissue sections were compared with regard to morphology, presence of EcPV2 DNA, presence and location of EcPV2 gene transcripts and expression of epithelial, mesenchymal and tumor/proliferation markers. Raft cultures from perilesional skin harboring only a few EcPV2-positive (EcPV2+) cells accurately recapitulated the differentiation process of normal skin, whilst rafts from EcPV2+ penile plaques were structurally organized but showed early hyperplasia. Rafts from EcPV2+ SCCs exhibited pronounced hyperplasia and marked dysplasia. Raft levels of EcPV2 oncogene transcription (E6/E7) and expression of tumor/proliferation markers p53, Ki67 and MCM7 expression positively correlated with neoplastic progression, again reflecting the natural situation. Three-dimensional raft cultures accurately reflected major features of corresponding ex vivo material, thus constituting a valuable new research model to study EcPV2-induced carcinogenesis.


© University of Veterinary Medicine ViennaHelp and DownloadsAccessibility statement