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Type of publication: Diploma Thesis
Type of document:

Year: 2011

Authors: Ostermeier, Sophia

Title: Aktivitäten der induzierbaren Hämoxygenase in Leber und Niere bei Tieren mit akuten systemischen Entzündungsreaktionen.

Other title: Activities of the inducible heme oxygenase in liver and kidney in animals with acute systemic inflammatory responses

Source: Diplomarbeit, Vet. Med. Univ. Wien, pp. 66.


Advisor(s):

Duvigneau Catharina

Reviewer(s):
Gemeiner Manfred

Vetmed Research Units:
Institute for Medical Biochemistry


Graduation date: 23.02.12


Abstract:
The enzyme heme oxygenase is presently the focus of numerous scientific research projects and is meanwhile considered a cytoprotective enzyme, making it extremely important for medicine. One goal of this thesis was to adapt the HO-Assay for measurement of HO activity in tissues of the pig. For this purpose the Assay should be optimized, allowing a precise and sensitive measurement of the enzyme activity with the lowest possible error rate. While using a BR dilution series, it was detected, that not all of the BR, a sample contains, can be extracted into the chloroform-phase, which is used for the photometric measurement. This loss was due to the presence of protein, can be quantified and can now be used to correct the obtained results. In addition, the effect of the HO-inhibitor ZnIXpp has been proofed and shown that the formation of BR in pigs, as described for the rat, is specific, and therefore the assay is suitable for measuring the HO activity. For the measurements obtained, it did not matter, as the tissue was previously diluted. In addition, it was confirmed that the BVR activity is not a limiting factor for the HO-Assay. However, it was shown that the HO activity less strongly increases with increasing concentrations of protein. This can be attributed to the interaction of BR with the proteins and its reduced extractability. So it must be taken out, either to work with constant amounts of protein in an assay, or to calculate the effect, is caused by the amount of protein, by using a correction formula.


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