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Selected Publication:

Type of publication: Baccalaureate Thesis
Type of document:

Year: 2015

Authors: Virgolini, Kathrin

Title: Optimierung und Anwendung eines Assays für die Bestimmung der Häm-degradierenden Enzymaktivitäten mit besonderer Berücksichtigung der Biliverdinreduktase in humanen Leukozytenpräparationen von Patienten mit SIRS und Sepsis.

Other title: Optimisation and application of an Assay for the determination of heme degradating enzyme activities with special focus on biliverdin reductase in preparations of human leukocytes of patients with SIRS and sepsis

Source: Bakkalaureatsarbeit, Vet. Med. Univ. Wien, pp. 56.


Advisor(s):

Duvigneau Catharina

Reviewer(s):
Schwendenwein Ilse

Vetmed Research Units:
Institute for Medical Biochemistry


Graduation date: 27.11.15


Abstract:
Biliverdin reductases (BVR) as well as hem oxygenase (HO) have a main function in the degradation pathway of hem proteins. BVR and HO are important for the protection against oxidative stress and also for cell signalling. The cytoprotective role of HO and BVR is believed to be mediated through the heme degeneration products biliverdin (BV) and bilirubin (BR). In endothelial cells it was shown that an unregulated HO-1 expression inhibits the activation of the endothelia and prevents an increase of leukocyte adhesion on the endothelium through oxidative stress. These effects are attributed to the heme degradation products BR and carbon monoxide. Due to the high concentration of BR the recycling of BV to BR through the BVR may have a special function in the vasculature as an endogen antioxidative system. As part of inflammatory challenge there is an increase in HO-activity in the endothelial cells and monocytes. Increased activity of heme degradation enzymes in the circulating leukocytes can activate endothelia through the BR production. Nobody has yet evaluated if there is a difference of the HO and the BVR-activity in leukocytes of Sepsis and SIRS (systemic inflammatory response syndrome). Therefore the following questions arose: - How the processing of the frozen leukocyte pellets has to be optimized to reproducible quantify HO and BVR activities. - Is there a difference in HO and BVR activities at Sepsis and SIRS or healthy probands?


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