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Publication type: Doctoral Thesis

Year: 2008

Author(s): Wenzel, N

Title: Veränderungen der Wasserstoffexhalation während einer Weideperiode und Einfluss der verschiedenen Wachstumsstadien von Weideaufwuchs auf die enterale mikrobielle Fermentation von Pferden.

Source: Dissertation, Vet. Med. Univ. Wien, pp. 165.


Advisor(s):

Van Den Hoven Rene
Zentek Jürgen


Abstract:
The aim of this study was to investigate the influence of pasture on enteric microbial activity by the aid of a hydrogen breath test and additional in vitro trials. Feed analyses included crude nutrients as well as the fructan content in pasture. The expired hydrogen was measured in eight horses, 3 times a day, each 14 days by the aid of a breath test, the LactoFAN®. Pasture was collected on each sampling day and weather conditions were recorded. Effects of temperature recorded by the meteorological station Grub were analyzed. In vitro-trials were performed. With the long-term incubation system pH and gas production were measured and samples were taken for later quantification of ammonia, lactate and short-chain fatty acids. In addition fermentative gas production was measured over 24 hours using a shortterm incubation system. At the last measuring day the highest breath hydrogen concentrations were measured. The hydrogen concentrations in the morning were lower, the evening values higher. Crude fiber, crude protein and fructan content correlated with the breath hydrogen concentration. Concerning the pH values measured in the long-term fermentation system a significant difference was found between the control fermenter and the fermenters which were fed with pasture. The ammonia content was significantly different at the sampling points. A significantly higher quantity of short chain fatty acids was found in the fermenters with pasture from june compared with collected at other time points. Gas production was highly variable without uniform trend in. Pasture quality appeared to influence the enteral microbial fermentation in horses. The hydrogen sensor appeared to be a simple and practicable tool for measuring hydrogen exhalationt in horses.

Keywords:
horses / hydrogen / exhalation / breath test / pasture / feed analysis / in vitro


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