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Publication type: Doctoral Thesis

Year: 2009

Author(s): Hollmann, Manfred

Title: Untersuchungen über die Schimmelpilze der Ordnung Mucorales in havarierten Grassilagen und Ganzpflanzenmaissilagen und ihre Mykotoxine.

Source: Dissertation, Vet. Med. Univ. Wien, pp. 130.


Advisor(s):

Böhm Josef

Vetmed Research Units:
Institute of Animal Nutrition and Functional Plant Compounds


Abstract:
Mucorales are the largest group of the Zygomycetes, encompassing such important genera as Absidia spp., Mucor spp. and Rhizopus spp. These are fast-growing fungi, which are important as fermentation agent for food and used extensively in the biotechnology for enzymes. Mucorales are regarded as the aetiological agents of Mucormycosis. Their capabilities to produce mycotoxins are not profoundly investigated, in contrast to those of the fungi from the genera Penicillium spp., Aspergillus spp. or Fusarium spp. The aim of this study was to isolate and identify fungi of the order Mucorales and investigate mycotoxin production. Twelve samples of visibly moulded grass silage and eight samples of damaged whole crop maize silage were analysed. Malt extract agar plates were used for sub cultivation. Three fungal species of the order Mucorales were isolated from grass silage, which were identified by their macro- and micro- morphology as Absidia corymbifera, Mucor circinelloides and Rhizopus stolonifer. The cytotoxicity of Mucor circinelloides extract was analysed using the cytotoxicity test(MTT assay). The result of the cytotoxicity assay showed a low cytotoxicity for Mucor circinelloides. Additionally extracts from Absidia corymbifera, Mucor circinalloides and Rhizopus stolonifer were tested for mycotoxins-production using LC/MS/MS-based multimycotoxin method. 3-nitropropionic (816 ng/kg) acid was detectid in the culture extract of Mucor circinelloides.

Keywords:
Mucorales / Mucor circinelloides / Silage / MTT-assay / 3-Nitropropionic acid


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