The aim of this study was to test the hypothesis, whether 10 mg artificially produced Deoxynivalenol (DON) per kg feed effects concentrations of retinol, [alpha]- and [gamma]-tocopherol in liver and small intestinum of broilers. For this, broiler diets were formulated containing Deoxynivalenol-contaminated wheat or control wheat. Vitamin supplementation followed international feeding standards. After six weeks, performance data (body weight, weight gain, feed intake, feed conversion) were measured and compared to literature. Based on HPLC methods, retinol, [alpha]- and [gamma]-tocopherol were analyzed in liver, duodenum and jejunum. Former studies showed first clinical symptoms starting at 16 mg DON per kg feed (feed refusal, reduced weight gain, decreased relative and absolute liver weight, increased relative and absolute gizzard weight). At 5 mg DON per kg feed first negative effect on glucose transport in duodenum is reported, at 10 mg DON the same can be seen in the jejunum. Performance data of the current study tended to result in positive influence of the trial group. Significant positive influence of DON could be found in jejunum ([alpha]-tocopherol, p =0,015) and liver ([gamma]-tocopherol, p = 0,045). Retinol tended to result in lower concentrations in the trial group. According to the results, 10 mg artificially produced DON per kg feed seems to influence positively performance data and tocopherols, but a negative effect on storage of retinol. Although concentration of DON was just 38 % lower than boundary value to clinical signs, the antioxidative status of the investigated parts of the gastrointestinal system was only fractional negatively influenced.