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Publication type: Baccalaureate Thesis

Year: 2016

Author(s): Popovova, Natalie

Title: Pilotstudie über Nachweis vom Allicinmetaboliten im Pferdeschweiß nach Fütterung eines Zusatzfuttermittles".

Other title: Proof of allicinmetabolites in horse sweat after feeding a dietary supplement - Pilotstudy

Source: Bakkalaureatsarbeit, Vet. Med. Univ. Wien, pp. 40.


Iben Christine

Novak Johannes

Vetmed Research Units:
Institute of Animal Nutrition and Functional Plant Compounds

The following pilot study is a close analysis of sweat, which has been extracted from horses fed with a dietary supplement containing 25 % of garlic powder. The sweat samples examined in this study have been obtained from 10 Quarter Horses that were chosen as suitable for the study after fulfilling a number of criteria, such as, for example the common similarity in sweat secretion. The objective of this study is the confirmation of the metabolites of allicin after feeding the horses with a supplement “Garlic Special“ produced by the company MIKROP Čebín a.s.. The first samples were taken before the launch of the project. The following samples were extracted after a three-week feeding process. The dosage of the used supplement was indicated on the product. The accurate feeding time had to be inquired personally at the producer of the garlic powder containing feed. After extraction, the samples were frozen. In order to proceed with the analysis procedure, the samples have been subsequently thawed, mixed with a solvent (Dichlormethan), cleaned and finally analyzed through GC-MS. There were no allicinmetabolites to see in any of the samples after evaluation of the gas chromatograms. In order to improve the quantitative analyzability, a pure garlic sample has been prepared. This sample was used as a positive control of the applied method. After the analysis of this sample, clear peaks of dithiins could be observed on the chromatogram. These sulfur compounds are one of the odor materials of garlic. The negative result of sweat samples resulted into an additional analysis of the feed supplement itself. This step has not been taken earlier, because the premise of the study was based primarily on the statement of the producer. No odor materials or other sulfur compounds were found in the pure sample of the supplement. The positive outcome of the pure garlic sample, where the odor materials were found, leads to a conclusion that the applied method is suitable for this kind of analysis. Consequently, the negative outcomes of the sweat samples must be depended on other aspects. To verify the outcomes of this study, it is necessary to carry out an additional trial. This experiment would require that the horses have to be fed with a fudder or dietary supplement in which the allicinmetabolites can be analyzed using the selected an alytic procedure. However, it is important to pay attention to the toxic potential of garlic for horses.

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