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Selected Publication:

Type of publication: Doctoral Thesis
Type of document:

Year: 2016

Authors: Sazmand, Alireza

Title: Molecular identification of vector-borne parasites in blood of camels (Camelus dromedarius) of Iran.

Other title: Molekulare Identifikation vektorübertragener Parasiten im Blut von Kamelen (Camelus dromedarius) aus dem Iran

Source: Dissertation, Vet. Med. Univ. Wien, pp. 103.


Advisor(s):

Joachim Anja

Reviewer(s):
Franz Sonja

Vetmed Research Units:
Institute of Parasitology


Abstract:
Dromedary camels are susceptible to a wide range of pathogens and act as a carrier or reservoir for the transmission of several transboundary animal diseases and zoonoses, but little is known about clinical or direct diagnostic proof of such pathogens by molecular techniques. The aim of this project was to obtain information on the occurrence, molecular characteristics and phylogeny of hemoparasites of camels in Iran. Jugular vein blood samples of 200 apparently healthy camels were taken from June to July 2014. From each sample, thin blood smears were prepared and stained with Giemsa for light microscopic examination. Also drops (approximately 100 μl) of EDTA blood were spotted on Whatman FTA® Elute sample collection cards and air dried. Disks of spotted blood were cut out from the FTA® cards and genomic DNA was extracted with the QIAamp® DNA Blood Mini Kit according to manufacturer’s instructions. PCR amplifications and sequencing was carried out for the detection of filaroid helminths, piroplasms and trypanosomes, followed by sequence analysis of positive samples. In light microscopy, helminths microfilariae were detected in four, and trypomastigote forms of Trypanosoma spp. in one of the camels. Using PCR and sequencing, 16 out of 200 (8 %) samples were positive for Dipetalonema evansi, including those positive by microscopy as well as one positive sample each with Theileria annulata and Trypanosoma evansi. Filter papers proved to be sensitive for camel hemoparasites’ epidemiological studies. To sum up, the results of this study add to the current knowledge on vector-borne diseases of camels. The subclinical nature of hemoparasitic infections in camels of the studied area was also shown which alerts their role as reservoirs of parasites’ transmission. Iranian one-humped camels could be described as hosts for Th. annulata, and they were confirmed as hosts for Tr. evansi. The availability of specific DNA sequences for D. evansi and their phylogenetic analysis now enable large-scale studies on its possible vectors in endemic areas by molecular typing. Further studies including experimental infections of piroplasmosis in camels will shed light on the clinical importance of the pathogens. Trypanotolerance of Iranian cattle and other livestock in an endemic area for Tr. evansi can be looked at in-depth for selection of proper breeds.

Keywords:
camel / dromedary / Iran / hemoparasites / vector-borne diseases / Dipetalonema / Theileria /Trypanosoma / PC


Publication(s) resulting from University thesis:

Sazmand, A; Eigner, B; Mirzaei, M; Hekmatimoghaddam, SH; Harl, J; Duscher, GG; Fuehrer, HP; Joachim, A (2017): Molecular Identification of Hemoprotozoan Parasites in Camels ( Iran J Parasitol. 2017; 11(4):568-573
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Sazmand, A; Eigner, B; Mirzaei, M; Hekmatimoghaddam, S; Harl, J; Duscher, GG; Fuehrer, HP; Joachim, A (2016): Molecular identification and phylogenetic analysis of Dipetalonema evansi (LEWIS, 1882) in camels (Camelus dromedarius) of Iran. Parasitol Res. 2016; 115(4):1605-1610

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