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Publication type: Diploma Thesis

Year: 2010

Author(s): Büchel, Christoph

Title: Vergleich von vier kommerziell erhältlichen ELISA-Tests zum Nachweis von Antikörpern gegen PRRSV mit speziellem Augenmerk auf die Analyse von Ausreissern.

Source: Diplomarbeit, Vet. Med. Univ. Wien, pp. 52.


Advisor(s):

Ritzmann Mathias

Reviewer(s):
Rossmanith, Wigbert

Vetmed Research Units:
University Clinic for Swine


Abstract:
The porcine reproductive and respiratory syndrome (PRRS) is one of the most economically important pig diseases worldwide. The clinical symptoms are late abortion, dead born or weak piglets as well as respiratory symptoms in weaning and fattening pigs. Different monitoring and eradication procedures have been established to minimize economic losses. In the present thesis, 353 sera, which were sent to the Clinic for Swine at the University of Veterinary Medicine Vienna for routine diagnostics, and 128 sera from a PRRSV vaccination trial were evaluated. The results of four different commercially available ELISA tests (IDEXX HerdChek® PRRS 2XR (ID 2XR), INGEZIM® PRRS DR (ING DR), INGEZIM® PRRS UNIVERSAL (ING UNI) und CIVTEST SUIS PRRS E/S (HIPRA)) were analysed with regard to reproducibility and to corresponding or non-corresponding results. The suitability for their usage in the course of PRRS monitoring programs was also tested. Special attention was turned to the analysis of possibly false-positive samples or outliers. An outlier was defined as single positive result out of 12 samples per herd tested during the monitoring program by the ELISA ING 2XR, in which all other analysed samples were negative.


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