Wild boars proved to be susceptible to infections with Porcine Reproductive and Respiratory
Sndrome Virus (PRRSV) and Porcine Circovirus Type 2 (PCV-2). Data on the prevalence of
both viruses in wild boars are inconsistent and information about the quantitative viral loads is
not readily available. This study investigated the prevalences of PRRSV and PCV-2 in wild
boars from southwestern Baden-Wurtemburg, Germany based on the results from serological
and PCR testing of tissue samples.
Samples were collected from 203 wild boars (Sus scrofa) hunted in Baden-Wurtemburg,
Germany from NovemberJanuary 2008 and 2009. Samples from lung and the tonsil were
analyzed by quantitative polymerase chain reaction (qPCR) for porcine reproductive and
respiratory syndrome virus (PRRSV) -type 1 (European type) and -type 2 (American type). A
qPCR to detect porcine circovirus type 2 (PCV2)-specific genome was performed on tissue
homogenates including lung, tonsils, and inguinal lymph nodes. Serum samples were tested
for antibodies against PRRSV and PCV2 by enzyme-linked immunosorbent assay (ELISA).
No PRRSV was detected in any of the 203 samples and one sample had detectable antibodies
against PRRSV. We detected PCV2 in organ materials from 103 wild boars with a prevalence
of 50.7%. The number of wild boars positive for PCV2 by PCR varied according to the
population density of wild boars among woodlands. More positive samples were detected in
woodlands with a high density of wild boars. We found no correlation between the number of
PCV2-positive wild boars and the density of domestic pigs in the surrounding area. The
number of wild boars positive for antibodies against PCV2 by the INGEZIM Circovirus
IgG/IgM test kit was low (53 sera positive for IgG- and three sera positive for IgMantibodies)
in comparison to the higher positive results from the INGEZIM CIRCO IgG test
kit (102 positive and 12 inconclusive results).