The objective of this prospective study was to prospectively evaluate serum concentrations of feline pancreas-specific lipase (Spec fPL®) and abdominal ultrasonography in cats with potential pancreatitis caused by trauma due to high-rise syndrome (HRS). A total of 34 cats presented for high-rise syndrome during the period from March to October 2009 to the Clinic of Surgery and Ophthalmology were included in this study. All cats presented had serum collected for measurement of Spec fPL concentrations within 12 hours after the fall, as well as 24, 48 and 72 hours after first blood collection. A diagnostic cut-off value for pancreatitis of >5.4µg/L was applied. Further two abdominal ultrasound examinations were performed in each cat 48 hours apart from each other and pancreatic trauma was assessed using four defined criteria (each assigned one point for a cumulative score): hypoechoic or heterogenous echogenicity of the pancreatic parenchyma, hyperechoic mesentery, enlargement of the pancreas and peritoneal effusion. A total score of >=3 points was considered suggestive of traumatic pancreatitis. Evidence for traumatic pancreatitis was identified in nine cats based on serum Spec fPL concentrations and in eight cats based on aUS. A significant correlation was found in the Spec fPL concentrations between the days measured (p<=0.01). In addition higher concentrations were detected on the first two days of measurement. There was a significant difference in the cumulative ultrasound score between the first and second ultrasound examination (p<0.001). Comparison of Spec fPL concentrations and abdominal ultrasound results revealed a significant agreement for diagnosis of traumatic pancreatitis in this study (82%). According to this study Spec fPL concentrations above the diagnostic cut-off value for pancreatitis are frequently observed in cats with HRS within the first 12 hours after the fall; a repeated abdominal ultrasound examination within 48 hours might further improve detection of pancreatic lesions after trauma.